CALL FOR PAPERS Updates on Mapping Quantitative Trait Loci QTL mapping in outbred populations: successes and challenges
نویسنده
چکیده
Solberg Woods LC. QTL mapping in outbred populations: successes and challenges. Physiol Genomics 46: 81–90, 2014. First published December 10, 2013; doi:10.1152/physiolgenomics.00127.2013.—Quantitative trait locus (QTL) mapping in animal populations has been a successful strategy for identifying genomic regions that play a role in complex diseases and traits. When conducted in an F2 intercross or backcross population, the resulting QTL is frequently large, often encompassing 30 Mb or more and containing hundreds of genes. To narrow the locus and identify candidate genes, additional strategies are needed. Congenic strains have proven useful but work less well when there are multiple tightly linked loci, frequently resulting in loss of phenotype. As an alternative, we discuss the use of highly recombinant outbred models for directly fine-mapping QTL to only a few megabases. We discuss the use of several currently available models such as the advanced intercross (AI), heterogeneous stocks (HS), the diversity outbred (DO), and commercially available outbred stocks (CO). Once a QTL has been finemapped, founder sequence and expression QTL mapping can be used to identify candidate genes. In this regard, the large number of alleles found in outbred stocks can be leveraged to identify causative genes and variants. We end this review by discussing some important statistical considerations when analyzing outbred populations. Fine-resolution mapping in outbred models, coupled with full genome sequence, has already led to the identification of several underlying causative genes for many complex traits and diseases. These resources will likely lead to additional successes in the coming years.
منابع مشابه
QTL mapping in outbred populations: successes and challenges.
Quantitative trait locus (QTL) mapping in animal populations has been a successful strategy for identifying genomic regions that play a role in complex diseases and traits. When conducted in an F2 intercross or backcross population, the resulting QTL is frequently large, often encompassing 30 Mb or more and containing hundreds of genes. To narrow the locus and identify candidate genes, addition...
متن کاملMicrosatellite mapping of quantitative trait loci affecting carcass traits on chromosome 1 in half-sib families of Japanese quail (Coturnix japonica)
The objective of this study was to identify the quantitative trait loci (QTL) affecting carcass traits on chromosome 1 in Japanese quail. The populations comprised of 422 progeny in 9 half-sib families. Phenotypic data on carcass weight, carcass parts, and the internal organs were collected on 422 progeny. Nine half-sib families were genotyped for 8 microsatellite markers covering chromosomes 1...
متن کاملA random model approach to mapping quantitative trait loci for complex binary traits in outbred populations.
Mapping quantitative trait loci (QTL) for complex binary traits is more challenging than for normally distributed traits due to the nonlinear relationship between the observed phenotype and unobservable genetic effects, especially when the mapping population contains multiple outbred families. Because the number of alleles of a QTL depends on the number of founders in an outbred population, it ...
متن کاملQTL Express: mapping quantitative trait loci in simple and complex pedigrees
QTL Express is the first application for Quantitative Trait Locus (QTL) mapping in outbred populations with a web-based user interface. User input of three files containing a marker map, trait data and marker genotypes allows mapping of single or multiple QTL by the regression approach, with the option to perform permutation or bootstrap tests.
متن کامل